Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Gold Biotechnology Inc Bis-Tris 500 g
Bis-Tris, also known as bis-Tris methane, is a zwitterionic buffering agent used in biochemistry and molecular biology. It is a bis(2-hydroxyethyl)amine and Tris family buffer that is useful for a pH range of 5.8 – 7.2. Bis Tris is a common component of many buffer systems for various types of electrophoresis. Bis-Tris may form a complex with some common metals, such as Cu(II) and Pb(II), and formation constants should be taken into account when using this buffer in a solution containing metal ions. Bis-Tris can be used as a substitute for the highly toxic buffer cacodylate.
Boston Bioproducts Inc HBS-N Buffer (10X, pH 7.4) - 4x500ml
HBS-N Buffer (10X pH 7 4) - 4x500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Phosphate Buffer (1 M, pH 7.6) - 500ml
Sodium Phosphate Buffer (1 M pH 7 6) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Dulbecco's Phosphate Buffered Saline, DPBS (no Calcium, no Magnesium) - 2x1L
Bioprocess Grade - Dulbecco s Phosphate Buffered Saline DPBS (no Calcium no Magnesium) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Phosphate Buffered Saline, PBS (10X, pH 7.6) - 4L
Phosphate Buffered Saline PBS (10X pH 7 6) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 7.4) - 1L
Tris-HCl Buffer (1 M pH 7 4) - 1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc TAPS Buffer (1 M, pH 8.5) - 100ml
TAPS Buffer (1 M pH 8 5) - 100ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-HCl Buffer (1 M, pH 8.0) - 2x1L
Bioprocess Grade - Tris-HCl Buffer (1 M pH 8 0) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Sodium Cacodylate Buffer (0.1 M, pH 6.5) - 100ml
Sodium Cacodylate Buffer (0 1 M pH 6 5) - 100ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffer (1 M, pH 7.5) - 2x1L
Bioprocess Grade - HEPES Buffer (1 M pH 7 5) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc M9 Minimal Salts Solution - 2x1L
M9 Minimal Salts Solution - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Tris-Base (2 M) - 2x1L
Bioprocess Grade - Tris-Base (2 M) - 2x1L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc HEPES Buffered Saline (5X, pH 7.4) - 500ml
HEPES Buffered Saline (5X pH 7 4) - 500ml (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc MES-SDS Buffer (20X) - 4L
MES-SDS Buffer (20X) - 4L (For Research Use and Further Manufacturing Only)
Boston Bioproducts Inc Bis-Tris Transfer Buffer (20X) - 1L
Bis-Tris Transfer Buffer (20X) - 1L (For Research Use and Further Manufacturing Only)