Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
Apexbio Technology LLC Annexin V-HF647/PI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes PI is a dye which specifically binds double-stranded DNA and emits red fluorescence under normal circumstances it cannot penetrate intact cell membranes However in late apoptotic and dead cells PI penetrates the cellular membrane staining the nuclei Dual staining with Annexin V-HF647 and PI enables clear differentiation between early apoptotic late apoptotic and dead cells
Apexbio Technology LLC Annexin V-HF647/DAPI Apoptosis Kit 20assays
Annexin V is a Ca2 -dependent phospholipid-binding protein that specifically binds to phosphatidylserine (PS) Normally PS is localized on the inner leaflet of the cell membrane lipid bilayer During the early stages of apoptosis PS translocates from the inner leaflet to the outer surface of the cell membrane exposing it to the extracellular environment Due to Annexin V s high affinity for PS fluorescently labeled Annexin V (HF647) can be used as a fluorescent probe to detect apoptotic cells through flow cytometry or fluorescence microscopy with staining taking only 10-20 minutes DAPI specifically binds double-stranded DNA producing blue fluorescence and normally cannot penetrate intact cell membranes However during late apoptosis or in dead cells DAPI can pass through compromised membranes to stain the nucleus Concurrent staining with Annexin V-HF647 and DAPI allows differentiation of cells at different stages of apoptosis and identification of dead cells
Alkali Scientific CellPro™ PBS Buffer, 1X, p.H 7.2, 1L, 1 Liter, 10/Case
CellPro™ PBS Buffer, 1X pH 7.2, 1 Liter Phosphate-buffered saline (PBS) is a balanced salt solution that is used for a variety of cell culture applications, such as washing cells before dissociation, transporting cells or tissue samples, diluting cells for counting, and preparing reagents. PBS is formulated without calcium and magnesium for rinsing chelators from the culture before cell dissociation. Concentrated forms of Gibco™ PBS may require pH adjustment at the time of dilution.
Bioworld ACES, 100 g
ACES, High Purity Grade N-[carbamoylmethyl]-2-Aminoethane-sulfonic acidpKa: (20°C): 6.8+0.1Appearance: White crystalsHS Code : 29241900Melting Point: >220 °C (dec.)(lit.)PH Value: 6.1-7.5
Teknova 20 x MOPS/SDS Running Buffer. 500mL, Sterile. 8 pack.
MOPS (3-(N-morpholino)propanesulfonic acid) is a Good’s buffer and has a pKa of 7.01, making it an ideal buffer for many biological systems at near-neutral pH. MOPS can be used in cell culture media and biopharma buffer formulations as well as in the purification of antibodies, peptides, proteins and blood components. It is nontoxic to cell culture lines.
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 1 Kg](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659877.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 1 Kg
PIPES Sodium Salt (Piperazine N,N' bis(2-ethanesulfonic acid) sodium salt) is a laboratory-grade biochemical reagent supplied in a 1 kg volume. PIPES is a widely used buffer in biochemical and molecular biology applications. It maintains a stable pH in a range of 6.1 to 7.5, making it ideal for use in studies requiring stable buffering at physiological pH. It is commonly used in protein electrophoresis, enzyme assays, and RNA research. The sodium salt form of PIPES provides excellent solubility and a low ionic strength, minimizing interference in sensitive experiments. This reagent is also used in cell culture media and biological research where precise pH control is essential for reliable results.
Hampton Research HR2-577/1.0 M HEPES sodium - 100 ml
HR2-577/1.0 M HEPES sodium - 100 ml. Please note items from this supplier are non-returnable.
Sigma Aldrich Fine Chemicals Biosciences SIGMA ALDRICH FINE CHEMICALS BIOSCIENCES
NC4005335 HEPES EMPROVE EVOLVE 100G
Medchemexpress LLC 4-Morpholinepropanesulfonic acid, sodium salt (1:1) | 71119-22-7 | 99.8% | 50 G
MOPS sodium salt is a buffering agent utilized in biological applications. It effectively maintains the pH of mammalian cell culture media. Additionally, MOPS sodium salt has been shown to significantly enhance the thermal stability of BSA (Bovine Serum Albumin).
- Buffering agent for biological applications.
- Maintains the pH of mammalian cell culture media.
- Significantly enhances the thermal stability of BSA.
![Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 250 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/5000659773.jpg-250.jpg)
Alkali Scientific PIPES Sodium Salt [Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt], 250 Grams
PIPES Sodium Salt (Piperazine N,N' bis(2 ethanesulfonic acid) sodium salt) is a high-purity biochemical reagent provided in a 250G pack. It is widely used as a buffering agent in biological and biochemical assays due to its excellent stability in a wide pH range. The sodium salt form of PIPES is ideal for maintaining stable pH conditions in sensitive biological environments. It is commonly used in molecular biology, protein analysis, and enzyme assays, as it does not interfere with biological systems. PIPES Sodium Salt is also used in chromatographic applications and as a mobile phase additive in high-performance liquid chromatography (HPLC).
Alkali Scientific ADA, [N (Carbamoylmethyl) iminodiacetic acid), 100 G
ADA (N-(Carbamoylmethyl) iminodiacetic acid) is a biochemical reagent available in a 100g bottle. This buffering agent is commonly used in laboratory settings for maintaining a stable pH in aqueous solutions. ADA is often employed in chromatography, enzyme assays, and protein purification where precise pH control is essential. It works effectively in various biochemical and molecular biology applications, providing a stable environment for enzymes and proteins. The 100g size is ideal for research laboratories and industrial applications requiring high-quality reagents for pH regulation and buffering in biochemical processes.
